Browsing by Author "Yildirim, Ender"

Now showing 1 - 13 of 13

Citation - WoS: 43
Citation - Scopus: 54
A normally closed electrostatic parylene microvalve for micro total analysis systems
(Elsevier Science Sa, 2012) Yildirim, Ender; Arikan, M. A. Sahir; Kulah, Haluk; 120121; Makine Mühendisliği
This paper presents an electrostatically actuated, normally closed microvalve for parylene microfluidics. The proposed valve structure isolates the fluid from the electric field, and hence results in relatively low actuation potentials (<60 V) irrespective of the working fluid. Hereby, the microvalve solves electrolysis or electrode shielding problems observed in electrostatic actuation in micro total analysis systems. To investigate leakage properties, microvalves were tested under pressurized flow with de-ionized (DI) water. No detectable leakage ratio was observed up to 20 kPa inlet pressure, due to the unique semicircular valve seat design. It was shown that the valve seat could be reconfigured to enable sealing at various pressure levels for different applications. (C) 2012 Elsevier B.V. All rights reserved.
Citation - WoS: 7
Citation - Scopus: 8
A novel zero-dead-volume sample loading interface for microfluidic devices: flexible hydraulic reservoir (FHR)
(Iop Publishing Ltd, 2018) Hatipoglu, Utku; Cetin, Barbaros; Yildirim, Ender; 158278; 31835; Makine Mühendisliği
Infusing minute amounts of valuable liquids such as samples to microfluidic chips by using common pumping schemes such as syringe pumps often result in an excessive dead-volume. We present a simple yet effective sample loading interface, which helps by pumping the sample to the chip by using the hydraulic pressure generated by the syringe pump. Results show that sample volumes as low as 25 mu l can be delivered at flow rates ranging between 10-30 mu l min(-1). Maximum dead volume ratio was observed to be 3% when infusing 200 mu l of sample at 10 mu l min(-1).
Citation - WoS: 12
Citation - Scopus: 13
A Reconfigurable Microfluidic Transmitarray Unit Cell
(Ieee, 2013) Erdil, Emre; Topalli, Kagan; Zorlu, Ozge; Toral, Taylan; Yildirim, Ender; Kulah, Haluk; Civi, Ozlem Aydin; 31835); Makine Mühendisliği
This paper presents a novel microfluidics based approach to develop a reconfigurable circularly polarized transmitarray unit cell. The unit cell comprises double layer nested split ring slots formed as microfluidic channels that can be filled by fluids. Split regions in the slots are realized by injecting liquid metal into the channels. Beam steering is obtained by implementing rotational phase shifting via manipulating the liquid metal in the slots. X-band unit cell prototypes are fabricated on glass substrate carrying a patterned metal film, and the slot channels are formed by Polydimethylsiloxane (PDMS) using soft lithography techniques.
Citation - WoS: 5
Citation - Scopus: 5
Analysis and testing of a contraction-and-expansion micromixer for micromilled microfluidics
(Springer Heidelberg, 2017) Yildirim, Ender; 31835; Makine Mühendisliği
In this paper, numerical analysis and experimental investigation of a micromixer, which was specifically designed for microfluidic devices fabricated by micromilling, is presented. The mixer is composed of series of contractions and expansions in zigzag arrangement along a mixing channel. Mixers, fabricated by micromilling on polymethylmethacrylate (PMMA), were tested with %0.1 Ponceau 4R red food dye solution and distilled water. According to experiment results, over 70% mixing efficiency could be obtained for the flows with Reynolds number (Re) greater than 40. It was also numerically shown that by increasing the number of successive contractions and expansions, it could be possible to achieve over 80% mixing efficiency when Re = 55 for the species with diffusion coefficient of 5 x 10(-9) m(2)/s. Although the micromixer was specifically designed for micromilling, it is expected that the mixer can be useful in any microfluidic device fabricated by any other technique.
Citation - WoS: 15
Citation - Scopus: 17
Electrostatic energy harvesting by droplet-based multi-phase microfluidics
(Springer Heidelberg, 2012) Yildirim, Ender; Kulah, Haluk; 120121; Makine Mühendisliği
This paper presents an energy scavenging technique, merging microfluidics with electrostatic energy harvesting. The method employs droplet-based microflow of two phases with different electrical permittivities, resulting in a capacitance change across the microchannel, to harvest electrical energy. The technique is implemented on 3 mm wide, 1 mm deep minichannels. It is shown that 0.4 nW can be harvested using a single electrode pair, with air and water as the two phases flowing at 1 ml/min. The generated power can be increased significantly by microscale implementation, where the number of electrodes can also be increased for further improvement.
Citation - WoS: 11
Citation - Scopus: 15
Fast fluorometric enumeration of E. coli using passive chip
(Elsevier, 2019) Kasap, Esin Nagihan; Dogan, Uzeyir; Cogun, Ferah; Yildirim, Ender; Boyaci, Ismail Haklu; Cetin, Demet; Ertas, Nusret; Makine Mühendisliği
In this report, a passive microfluidic chip design was developed for fast and sensitive fluorometric determination of Escherichia coli (E. coli) based on sandwich immunoassay. Initially, magnetic nanoparticles (MNPs) and chitosan modified mercaptopropionic acid capped cadmium telluride (CdTe) quantum dots (QDs) were functionalized with E.coli specific antibody to form a sandwich immunoassay with the E. coli. The magnetic separation and preconcentration of the E.coli from the sample solution was performed in the vial. Conjugation of QDs to the magnetically captured E. coli and washing were performed using a passive type of microchip. The microfluidic chip consists of four microchambers connected to each other by microchannels which act as capillary valves. Signal measurement was performed at the last chamber by using a hand-held spectrofluorometer equipped with a fiber optic reflection probe. The selectivity of the method was tested with Enterobacter aerogenes (E. aerogenes) and Salmonella enteritidis (S. enteritidis), it was observed that these bacteria have no interference effect on E.coli determination. The calibration curve was found to be linear in the range of 10(1)-10(5) cfu/mL with a correlation coefficient higher than 0.99. The limit of detection was calculated as 5 cfu/mL. The method was successfully applied to spiked tap and lake water samples. The results suggest that the developed method is applicable for on-site E. coli detection and offers several advantages such as large dynamic range, high sensitivity, high selectivity and short analysis time.
Citation - WoS: 0
Citation - Scopus: 0
Fluorescent on-chip imager by using a tunable absorption filter
(Ieee, 2017) Arpali, Caglar; Yildirim, Ender; Arpali, Serap Altay; 20809; 31835; 51304; Makine Mühendisliği; Mekatronik Mühendisliği; Elektronik ve Haberleşme Mühendisliği
Citation - WoS: 6
Citation - Scopus: 10
Implementation and characterization of an absorption filter for on-chip fluorescent imaging
(Elsevier Science Sa, 2017) Yildirim, Ender; Arpali, Caglar; Arpali, Serap Altay; 31835; 20809; 51304; Makine Mühendisliği; Mekatronik Mühendisliği; Elektronik ve Haberleşme Mühendisliği
Here we present fabrication and characterization of an absorption filter with superior roll-on properties and precisely tunable cut-off wavelengths for fluorescent imaging applications in lab-on-a-chip systems. The filters were fabricated by spinning dye doped photopolymer (Orasol Yellow in Norland Optical Adhesive 60) on glass substrates. The fabrication technique allowed us to precisely tune the cut-off wavelength of the filters. We showed that filters with different cut-off in the range of 386 nm-504 nm could be obtained simply by controlling the settling time before spinning. The filters exhibited a steep roll-on from stopband to passband at the cut-off. Transmission in the stopband was observed to be maximum 3% while it was almost constant at 100% in the passband within the range of 220 nm-620 nm. On-chip use of the filters was also demonstrated for imaging particular fluorescent beads. (C) 2016 Elsevier B.V. All rights reserved.
Citation - WoS: 27
Citation - Scopus: 30
Investigation on replication of microfluidic channels by hot embossing
(Taylor & Francis inc, 2017) Cogun, Ferah; Yildirim, Ender; Arikan, M. A. Sahir; 31835; Makine Mühendisliği
In this study, effects of embossing temperature, time, and force on production of a microfluidic device were investigated. Polymethyl methacrylate (PMMA) substrates were hot embossed by using a micromilled aluminum mold. The process parameters were altered to observe the variation of replication rate in width and depth as well as symmetry of the replicated microfluidic channels. Analysis of variance (ANOVA) on the experimental results indicated that embossing temperature was the most important process parameter, whereas embossing time and force have less impact. One distinguishing aspect of this study is that, the channels were observed to be skewed to either side of the channel depending on the location of the protrusions on the mold. The mechanism of the skewness was investigated by finite element analysis and discussed in detail. Results showed that the skewness depends on the flow characteristics of the material and could be reduced by increasing the embossing temperature. The best replication rates were obtained at parameter settings of 115 degrees C, 10kN, and 8min for the molds with minimum 56 mu m wide features of 120 mu m depth. We also showed that the fabricated channels could be successfully sealed by solvent-assisted thermo-compressive bonding at 85 degrees C under 5.5kN force.
Citation - WoS: 11
Citation - Scopus: 14
Numerical study on effects of computational domain length on flow field in standing wave thermoacoustic couple
(Elsevier Sci Ltd, 2019) Mergen, Suhan; Yildirim, Ender; Turkoglu, Hasmet; Makine Mühendisliği
For the analysis of thermoacoustic (TA) devices, computational methods are commonly used. In the computational studies found in the literature, the flow domain has been modelled differently by different researchers. A common approach in modelling the flow domain is to truncate the computational domain around the stack, instead of modelling the whole resonator to save computational time. However, where to truncate the domain is not clear. In this study, we have investigated how the simulation results are affected by the computational domain length (I-d) when the truncated domain approach is used. For this purpose, a standing wave TA couple which undergoes a refrigeration cycle was considered. The stack plate thickness was assumed to be zero and the simulations were performed for six different dimensionless domain length (I-d/lambda) varying between 0.029 and 0.180. Frequency and Mach number were taken as 100 Hz and 0.01, respectively, and kept constant for all the cases considered. The mean pressure and the pressure amplitude were taken as 10 kPa and 170 Pa, respectively (Drive ratio of 1.7%). Helium was considered as the working fluid. To assess the accuracy of the simulation results, the pressure distributions across the domain were compared with that of the standing wave. In addition to the pressure variation, the effects of the domain length on the phase delay of the pressure and velocity waves along the stack plate were also investigated. The results showed that with the increasing I-d/lambda. ratio, the simulated pressure distribution compares better with the standing wave pressure distribution. With the lowest I-d/lambda ratio (0.029) considered, the difference between the amplitudes of the computed pressure distribution and theoretical standing wave pressure distribution was approximately 50 Pa. However, as I-d/lambda value increases, the simulation results approach to the theoretical standing wave pressure distribution better. The computational results obtained with Id/lambda = 0.132 and 0.180, were almost identical with standing wave acoustic field. Hence, it was concluded that the domain length has a significant effect on the accuracy of the computational results when the truncated domain approach is used. It was also observed that for a given TA device and operating parameters, there is a minimum I-d/lambda value for obtaining reliable results.
Citation - WoS: 22
Citation - Scopus: 21
Phaseguide assisted liquid lamination for magnetic particle-based assays
(Royal Soc Chemistry, 2014) Phurimsak, Chayakom; Yildirim, Ender; Tarn, Mark D.; Trietsch, Sebastiaan J.; Hankemeier, Thomas; Pamme, Nicole; Vulto, Paul; 31835; Makine Mühendisliği
We have developed a magnetic particle-based assay platform in which functionalised magnetic particles are transferred sequentially through laminated volumes of reagents and washing buffers. Lamination of aqueous liquids is achieved via the use of phaseguide technology; microstructures that control the advancing air-liquid interface of solutions as they enter a microfluidic chamber. This allows manual filling of the device, eliminating the need for external pumping systems, and preparation of the system requires only a few minutes. Here, we apply the platform to two on-chip strategies: (i) a one-step streptavidin-biotin binding assay, and (ii) a two-step C-reactive protein immunoassay. With these, we demonstrate how condensing multiple reaction and washing processes into a single step significantly reduces procedural times, with both assay procedures requiring less than 8 seconds.
Citation - WoS: 27
Citation - Scopus: 31
Phaseguides as tunable passive microvalves for liquid routing in complex microfluidic networks
(Royal Soc Chemistry, 2014) Yildirim, Ender; Trietsch, Sebastiaan J.; Joore, Jos; van den Berg, Albert; Hankemeier, Thomas; Vulto, Paul; 31835; Makine Mühendisliği
A microfluidic passive valving platform is introduced that has full control over the stability of each valve. The concept is based on phaseguides, which are small ridges at the bottom of a channel acting as pinning barriers. It is shown that the angle between the phaseguide and the channel sidewall is a measure of the stability of the phaseguide. The relationship between the phaseguide-wall angle and the stability is characterized numerically, analytically and experimentally. Liquid routing is enabled by using multiple phaseguide with different stability values. This is demonstrated by filling complex chamber matrices. As an ultimate demonstration of control, a 400-chamber network is used as a pixel array. It is the first time that differential stability is demonstrated in the realm of passive valving. It ultimately enables microfluidic devices for massive data generation in a low-cost disposable format.
Citation - WoS: 28
Citation - Scopus: 31
SERS-Based Ultrafast and Sensitive Detection of Luteinizing Hormone In Human Serum Using A Passive Microchip
(Elsevier Science Sa, 2018) Gjergjizi, Belma; Cogun, Ferah; Yildirim, Ender; Eryilmaz, Merve; Selbes, Yesim; Saglam, Necdet; Tamer, Ugur; 3837; Makine Mühendisliği
Human luteinizing hormone (LH) is an important analyte for doping control analysis since it increases the athletic performance. However, traditional methods to detect LH have few disadvantages, such as long analysis duration, waste disposal problem and sample matrix effect. Addressing these problems, surface enhanced Raman spectroscopy based LH analysis using a passive microfluidic chip was developed and optimized. Antibody modified magnetic gold nanoparticles captured the LH and then, 4-aminothiophenol (4-ATP) labeled nanoparticles formed the sandwich immunoassay structure. The complex and the other reactions occurred in different chambers of the chip. The SERS signals of 4-ATP were recorded from the chamber and the system was shown to detect 0.036 IU L-1 in serum samples. The performance of the immunoassay was compared to all other methods and the proposed assay was the fastest analysis of LH without any problems associated with the sensitivity. The shorter analysis time was recorded because the chip enables the control of all reactions in one place and there was no requirement of a specialized laboratory. (c) 2018 Elsevier B.V. All rights reserved.