Pazarceviren, EnginErdemli, OzgeKeskin, DilekTezcaner, Aysen01. Çankaya Üniversitesi2020-03-182025-09-182020-03-182025-09-182017Pazarceviren, Engin...et al. (2017)."Clinoptilolite/PCL-PEG-PCL composite scaffolds for bone tissue engineering applications", Journal Of Biomaterials Applications, Vol.31, No.8, pp.1148-1168.0885-32821530-8022https://doi.org/10.1177/0885328216680152https://hdl.handle.net/123456789/12556Tezcaner, Aysen/0000-0003-4292-5856; Erdemli, Ozge/0000-0001-8606-8863; Pazarceviren, Ahmet Engin/0000-0001-5233-860XThe aim of this study was to prepare and characterize highly porous clinoptilolite/poly(epsilon-caprolactone)-poly(ethylene glycol)-poly(epsilon-caprolactone) composite scaffolds. Scaffolds with different clinoptilolite contents (10% and 20%) were fabricated with reproducible solvent-free powder compression/particulate leaching technique. The scaffolds had interconnective porosity in the range of 55-76%. Clinoptilolite/poly(epsilon-caprolactone)-poly(ethylene glycol)poly(epsilon-caprolactone) scaffolds showed negligible degradation within eight weeks and displayed less water uptake and higher bioactivity than poly(epsilon-caprolactone)-poly(ethylene glycol)-poly(epsilon-caprolactone) scaffolds. The presence of clinoptilolite improved the mechanical properties. Highest compressive strength (5.6 MPa) and modulus (114.84 MPa) were reached with scaffold group containing 20% clinoptilolite. In vitro protein adsorption capacity of the scaffolds was also higher for clinoptilolite/poly(epsilon-caprolactone)-poly(ethylene glycol)-poly(epsilon-caprolactone) scaffolds. These scaffolds had 0.95 mg protein/g scaffold adsorption capacity and also higher osteoinductivity in terms of enhanced ALP, OSP activities and intracellular calcium deposition. Stoichiometric apatite deposition (Ca/P = 1.686) was observed during cellular proliferation analysis with human fetal osteoblasts cells. Thus, it can be suggested that clinoptilolite/poly(epsilon-caprolactone)poly(ethylene glycol)-poly(epsilon-caprolactone) composite scaffolds could be promising carriers for enhancement of bone regeneration in bone tissue engineering applications.eninfo:eu-repo/semantics/closedAccessClinoptilolitePcl-Peg-PclParticulate LeachingSolvent-FreePowder CompressionBone Tissue EngineeringArticle10.1177/08853282166801522-s2.0-85018261814