Phurimsak, C.Yıldırım, EnderYildirim, E.Trietsch, S.J.Hankemeier, T.Tarn, M.D.Pamme, N.Vulto, P.Makine Mühendisliği2024-03-262024-03-262013Phurimsak, Chayakom...et all. "Phaseguide assisted liquid lamination for magnetic bead-based assays", 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 27-31 October 2013, Freiburg, Germany, pp. 1153-1155, 2013.9781632666246Agilent; Centre for Biological Signalling Studies (BIOSS); Forum for Applied Informatics and Microengineering (FAIM); HSG-IMIT; MicroTec Sudwest; Roche DiagnosticsWe demonstrate a simple, pump-free platform for performing rapid magnetic bead-based processes via their transfer through sequentially laminated liquid streams, made possible by the use of phaseguide technology. We have applied this strategy to two on-chip assays: (i) a streptavidin-biotin binding assay, and (ii) a sandwich immunoassay for the detection of C-reactive protein (CRP). Here, functionalized magnetic beads were pulled through alternating lanes of reagents and buffer solution, allowing multiple binding and washing processes to be reduced into a single step, significantly shortening procedural times compared to conventional multi-step bead-based assays.eninfo:eu-repo/semantics/closedAccessC-Reactive ProteinImmunoassaysLiquid LaminationMagnetic BeadsPhaseguidesConference Object2115311552-s2.0-84907310841N/AN/A