Phurimsak, ChayakomYıldırım, EnderTrietsch, Sebastiaan J.Hankemeier, ThomasTarn, Mark D.Pamme, NicoleVulto, Paul2024-03-262024-03-262013Phurimsak, Chayakom...et all. "Phaseguide assisted liquid lamination for magnetic bead-based assays", 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 27-31 October 2013, Freiburg, Germany, pp. 1153-1155, 2013.http://hdl.handle.net/20.500.12416/7748We demonstrate a simple, pump-free platform for performing rapid magnetic bead-based processes via their transfer through sequentially laminated liquid streams, made possible by the use of phaseguide technology. We have applied this strategy to two on-chip assays: (i) a streptavidin-biotin binding assay, and (ii) a sandwich immunoassay for the detection of C-reactive protein (CRP). Here, functionalized magnetic beads were pulled through alternating lanes of reagents and buffer solution, allowing multiple binding and washing processes to be reduced into a single step, significantly shortening procedural times compared to conventional multi-step bead-based assays.eninfo:eu-repo/semantics/closedAccessMagnetic BeadsPhaseguidesLiquid LaminationImmunoassaysC-Reactive ProteinConference Object11531155