Browsing by Author "Çoğun, Ferah"

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Chip-Based Immunomagnetic Separation of Human Chorionic Gonadotropin
(2016) Çoğun, Ferah; Yıldırım, Ender; Çiftçi, Hakan; Çağlayan, M. Gökhan; Somay Selbes, Yeşim; Çoğun, Ferah; Yıldırım, Ender; Tamer, Uğur
Comparison of different yield criteria in various deep drawn cups
(Springer, 2017) Çoğun, Ferah; Darendeliler, Haluk; 36624; 7702
The aim of the study is to evaluate the performance of three recent yield criteria namely; BBC2008-8p, Yld2003-8p, Hu2003 through the simulation of the hemispherical, cylindrical and square cup drawing processes by comparing the results with the ones obtained by using the von Mises criterion for the isotropic, kinematic and combined hardening and the Hill'48 criterion. For this purpose, two different sheet materials, SS304 stainless steel and DKP6112 steel, and various punch travels were used in the simulations and experimental phases of this study. The BBC2008-8p, Yld2003-8p and Hu2003 models were implemented to the ABAQUS software through the user material subroutine VUMAT. The thickness strain distributions obtained from the simulations were compared with the experimental results to analyze the validity of the three aforementioned criteria. Compared with the other models, the material behavior in deep drawing cases of this study is better predicted with more recent models, namely BBC2008-8p, Yld2003-8p and Hu2003, which include anisotropy parameters found from uniaxial and biaxial tension tests.
Effect of powder metallurgy Cu-B4C electrodes on workpiece surface characteristics and machining performance of electric discharge machining
(Sage Publications Ltd, 2016) Çoğun, Can; Cogun, Can; Esen, Ziya; Esen, Ziya; Genc, Asim; Çoğun, Ferah; Cogun, Ferah; Akturk, Nizami; 3837; 52373
The main aim of this study is to produce new powder metallurgy (PM) Cu-B4C composite electrode (PM/(Cu-B4C)) capable of alloying the recast workpiece surface layer during electric discharge machining process with boron and other hard intermetallic phases, which eventually yield high hardness and abrasive wear resistance. The surface characteristics of the workpiece machined with a PM/(Cu-B4C) electrode consisted of 20 wt% B4C powders were compared with those of solid electrolytic copper (E/Cu) and powder metallurgy pure copper (PM/Cu) electrodes. The workpiece surface hardness, surface abrasive wear resistance, depth of the alloyed surface layer and composition of alloyed layers were used as key parameters in the comparison. The workpiece materials, which were machined with PM/(Cu-B4C) electrodes, exhibited significantly higher hardness and abrasive wear resistance than those of machined with the E/Cu and PM/Cu. The main reason was the presence of hard intermetallic phases, such as FeB, B4C (formed due to the boron in the electrode) and Fe3C in the surface layer. The improvement of the surface hardness achieved for steel workpiece when using PM/(Cu-B4C) electrodes was significantly higher than that reported in the literature. Moreover, the machining performance outputs (workpiece material removal rate, electrode wear rate and workpiece average surface roughness (Ra)) of the electrodes were also considered in this study.
Fast fluorometric enumeration of E. coli using passive chip
(Elsevier, 2019) Çoğun, Ferah; Kasap, Esin Nagihan; Dogan, Uzeyir; Yıldırım, Ender; Cogun, Ferah; Yildirim, Ender; Boyaci, Ismail Haklu; Cetin, Demet; Ertas, Nusret
In this report, a passive microfluidic chip design was developed for fast and sensitive fluorometric determination of Escherichia coli (E. coli) based on sandwich immunoassay. Initially, magnetic nanoparticles (MNPs) and chitosan modified mercaptopropionic acid capped cadmium telluride (CdTe) quantum dots (QDs) were functionalized with E.coli specific antibody to form a sandwich immunoassay with the E. coli. The magnetic separation and preconcentration of the E.coli from the sample solution was performed in the vial. Conjugation of QDs to the magnetically captured E. coli and washing were performed using a passive type of microchip. The microfluidic chip consists of four microchambers connected to each other by microchannels which act as capillary valves. Signal measurement was performed at the last chamber by using a hand-held spectrofluorometer equipped with a fiber optic reflection probe. The selectivity of the method was tested with Enterobacter aerogenes (E. aerogenes) and Salmonella enteritidis (S. enteritidis), it was observed that these bacteria have no interference effect on E.coli determination. The calibration curve was found to be linear in the range of 10(1)-10(5) cfu/mL with a correlation coefficient higher than 0.99. The limit of detection was calculated as 5 cfu/mL. The method was successfully applied to spiked tap and lake water samples. The results suggest that the developed method is applicable for on-site E. coli detection and offers several advantages such as large dynamic range, high sensitivity, high selectivity and short analysis time.
Investigation on replication of microfluidic channels by hot embossing
(Taylor & Francis inc, 2017) Çoğun, Ferah; Cogun, Ferah; Yildirim, Ender; Yıldırım, Ender; Arikan, M. A. Sahir; 31835
In this study, effects of embossing temperature, time, and force on production of a microfluidic device were investigated. Polymethyl methacrylate (PMMA) substrates were hot embossed by using a micromilled aluminum mold. The process parameters were altered to observe the variation of replication rate in width and depth as well as symmetry of the replicated microfluidic channels. Analysis of variance (ANOVA) on the experimental results indicated that embossing temperature was the most important process parameter, whereas embossing time and force have less impact. One distinguishing aspect of this study is that, the channels were observed to be skewed to either side of the channel depending on the location of the protrusions on the mold. The mechanism of the skewness was investigated by finite element analysis and discussed in detail. Results showed that the skewness depends on the flow characteristics of the material and could be reduced by increasing the embossing temperature. The best replication rates were obtained at parameter settings of 115 degrees C, 10kN, and 8min for the molds with minimum 56 mu m wide features of 120 mu m depth. We also showed that the fabricated channels could be successfully sealed by solvent-assisted thermo-compressive bonding at 85 degrees C under 5.5kN force.
Microchip Based Determination of Bacteria by In Chip Sandwich Immunoassay International Multidisciplinary Symposium on Drug Research and Development
(2015) Çoğun, Ferah; Yıldırım, Ender; Yıldırım, Ender; Boyacı, İsmail Hakkı; Çetin, Demet; Suludere, Zekiye; Tamer, Uğur; Ertaş, Nusret
SERS-Based Ultrafast and Sensitive Detection of Luteinizing Hormone In Human Serum Using A Passive Microchip
(Elsevier Science SA, 2018) Çoğun, Ferah; Yıldırım, Ender; Yıldırım, Ender; Eryılmaz, Merve; Selbes, Yeşim; Sağlam, Necdet; Tamer, Uğur; 3837
Human luteinizing hormone (LH) is an important analyte for doping control analysis since it increases the athletic performance. However, traditional methods to detect LH have few disadvantages, such as long analysis duration, waste disposal problem and sample matrix effect. Addressing these problems, surface enhanced Raman spectroscopy based LH analysis using a passive microfluidic chip was developed and optimized. Antibody modified magnetic gold nanoparticles captured the LH and then, 4-aminothiophenol (4-ATP) labeled nanoparticles formed the sandwich immunoassay structure. The complex and the other reactions occurred in different chambers of the chip. The SERS signals of 4-ATP were recorded from the chamber and the system was shown to detect 0.036 IU L-1 in serum samples. The performance of the immunoassay was compared to all other methods and the proposed assay was the fastest analysis of LH without any problems associated with the sensitivity. The shorter analysis time was recorded because the chip enables the control of all reactions in one place and there was no requirement of a specialized laboratory. (c) 2018 Elsevier B.V. All rights reserved.
Simultaneous Detection of Two Different Bacteria Using QDs and MNPs
(2016) Çoğun, Ferah; Yıldırım, Ender; Çoğun, Ferah; Yıldırım, Ender; Çetin, Demet; Suludere, Zekiye; Boyacı, İsmail Hakkı; Ertaş, Nusret; Tamer, Uğur