Browsing by Author "Aksoy, H"

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Chemometric approach to simultaneous chromatographic determination of paracetamol and chlorzoxazone in tablets and spiked human plasma
(Taylor & Francis inc, 2006) Dinç, E; Baleanu, Dumitru; Ozdemir, A; Aksoy, H; Baleanu, D; 6981; 26096
New chemometric approaches were introduced into the high performance liquid chromatographic (HPLC) determination of chlorzoxazone (CZX) and paracetamol (PAR) in the tablets and spiked human plasma. These chemometric approaches contain the application of classical least squares (CLS), principle component regression (PCR), and partial least squares (PLS) calibrations to the multiwavelength HPLC peak area obtained by plotting the chromatograms at the five wavelengths. The multichromatograms were obtained by using the photodiode array detector at 225 (A), 240 (B), 255 (C), 270 (D), and 285 (E) nm. The algorithms of CLS, PCR, and PLS were applied to the multichromatogram data to construct the HPLC-CLS, HPLC-PCR, and HPLC-PLS calibrations. A mixture of acetonitrile and 0.1 M ammonium carbonate (60:40, v/v) on a Waters Symmetry (R) trademark C18 Column 5 mu m 4.6 x 250 mm at a flow rate of 0.8 mL/min was used as a mobile phase to separate and determine CZX and PAR in samples. Hydrochlorothiazide (IS) was used as an internal standard in this chromatographic separation. The HPLC chemometric calibrations were successfully applied to the quantitative analysis of the investigated drugs in commercial pharmaceutical preparation and spiked human plasma samples with high precision and accuracy.
Chemometric Determination of Naproxen Sodium and Pseudoephedrine Hydrochloride in Tablets by HPLC
(Pharmaceutical Soc Japan, 2006) Dinç, E; Baleanu, Dumitru; Özdemir, A; Aksoy, H; Üstündag, Ö; Baleanu, D; 6981; 26096; 105547
A new chemometric determination by high-performance liquid chromatography (HPLC) with photodiode array (PDA) detection was implemented for the simultaneous determination of naproxen sodium and pseudoephedrine hydrochloride in tablets. Three chemometric calibration techniques, classical least squares (CLS), principle component regression (PCR) and partial least squares (PLS) were applied to the peak area at multiwavelength PDA detector responses. The combinations of HPLC with chernometric calibration techniques were called HPLC-CLS, HPLC-PCR and HPLC-PLS. For comparison purposes the HPLC method called the classic HPLC method was used to confirm the results obtained from combined HPLC-chemometric calibration techniques. A good chromatographic separation between two drugs with losartan potassium as an internal standard was achieved using a Waters Symmetry (R) C18 Column 5 mu m 4.6 +/- 250 mm and a mobile phase containing 0.2 m acetate buffer and acetonitrile (v/v, 40 : 60). The multiwavelength PDA detection was measured at five different wavelengths. The chromatograms were recorded as a training set in the mobile phase. Three HPLC-chemometric calibrations and the classic-HPLC method were used to test the synthetic mixtures of naproxen sodium and pseudoephedrine hydrochloride in the presence of the internal standard. The HPLC-chemometric approaches were applied to real samples containing drugs of interest. The experimental results obtained from HPLC-chemometric calibrations were compared with those obtained by a classic HPLC method.