Browsing by Author "Dermis, Saadet"

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Citation - WoS: 12
Citation - Scopus: 15
Determination of Guaifenesin and Dextromethorphan in a Cough Syrup by Hplc With Fluorometric Detection
(Editura Academiei Romane, 2006) Ozdemir, Abdil; Baleanu, Dumitru; Aksoy, Halil; Dinc, Erdal; Baleanu, Dumitru; Dermis, Saadet; Matematik
A practical high-performance liquid chromatographic method with fluorometric detection for the determination of guaifenesin and dextromethorphan in cough syrup is described. The developed method contains simple, accurate and rapid sample preparation steps for the separation and simultaneous determination of active compounds in samples with existence of other excipients. Amilorid (AM) was used as an internal standard (IS) that has a fluorescence character in the working wavelength region. A good chromatographic separation of two drugs and IS was achieved on a Waters Symmetry(R) C18 Column 5 mu m 4.6 x 250 mm with mobile phase consisting of phosphate buffer (0.2 M, pH=2), acetonitrile and methyl alcohol (v/v, 62:23:15). Eluents were monitored by fluorescence detection at the excitation and emission wavelengths of 277 and 588 nm, respectively. Under the described conditions, the obtained calibration graphs are linear over the concentration range of 0.054.2 and 1.2-2.4 mg/mL for dextromethorphan and guaifenesin, respectively and the regression coefficients were greater than 0.999. The validation study was carried out fulfilling the ICH guidelines in order to prove that the new analytical method meets the fundamental criteria including selectivity, linearity, precision, accuracy and sensitivity.
Citation - WoS: 7
Citation - Scopus: 9
Simultaneous Spectrophotometric Determination of Chlordiazepoxide and Clidinium Bromide in Sugar Coated Tablets by Partial Least Squares, Principal Component Regression and Artificial Neutral Network
(Chiminform Data S A, 2006) Dinc, Erdal; Baleanu, Dumitru; Dermis, Saadet; Baleanu, Dumitru; Matematik
Multivariate spectrophotometric approaches based on namely partial least squares (PLS), principal component regression (PCR) and artificial neural network (ANN) were proposed for the simultaneous determination of chlordiazepoxide (CHP) and clidinium bromide (CLB) in sugar coated tablets without any chemical separation step. As an alternative approach, ANN was applied to obtain the calibration function between the training data matrix and its corresponding absorbance data matrix. A training set consisting of 24 mixture solutions in the concentration range of 0-25 mu g/mL CHP and 0-30 mg/mL was prepared in methanol and 0.1 M NaOH (60:40, v/v). PLS and PCR calibrations were constructed by using their chemometric algorithms dependent on the relationships training set and absorbance data matrix obtained in the spectral range of 210-400 nm. As a comparison approach, ANN was applied to the determination of the investigated drugs in their mixtures and the commercial pharmaceutical preparation to clarify interactions between the analyzed compounds and excipients in samples. The validation of these chemometric methods was carried out by analyzing the artificial mixtures containing CHP and CLB drugs. The mathematical treatments were performed by using Microsoft Excel, a neural network algorithm written in Matlab 7.0 and the PLS toolbox 3.5. These multivariate approaches were successfully applied to the real CHP and CLB samples. As a result, the proposed chemometric methods were found suitable for the quality control and routine analysis of two drugs in sugar coated tablets.