Browsing by Author "Ioele, Giuseppina"

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Fractional wavelet analysis for the simultaneous quantitative analysis of lacidipine and its photodegradation product by continuous wavelet transform and multilinear regression calibration
(AOAC International, 2006) Baleanu, Dumitru; Ragno, Gaetano; Ioele, Giuseppina; Baleanu, Dumitru; 6981
Fractional wavelet transform (FWT) was applied to the original absorption spectra of lacidipine (LAC) and its photodegradation product (LACD), and the resulting FWT spectra were processed by continuous wavelet transform (CWT) and multilinear regression calibration (MLRC) for the simultaneous quantitative analysis of both products in their binary mixtures. These methods do not require any chemical separation step and chemical complex reaction to obtain a detectable signal for the degradation product. By using the Mexican hat function, 2 calibration functions for LAC and LACD were obtained by measuring the CWT transformed signals at 416.1 nm for LAC and 414.6 nm for LACD, after FWT processing of the original absorption spectra. The calibration graphs were linear in the concentration range of 5.08-40.64 mu g/mL for LAC and 0.51-8.16 mu g/mL for LACD. The limit of detection and the limit of quantitation were found to be 0.289 and 0.956 mu g/mL for LAC and 0.036 and 0.118 mu g/mL for LACD, respectively. For comparison, the MLRC algorithm was applied to the linear regression functions for the individual drug and its photoproduct. In this approach, a set of linear regression functions was obtained from the relationship between concentrations and FWT signals in the wavelength range 411.0-412.4 nm. Both methods were applied to the quantitative evaluation of LAC and LACD in laboratory and pharmaceutical samples, and produced very satisfactory results
Fractional wavelet transform-continous wavelet transform for the quantification of melatonin and its photodegradation product
(Taylor & Francis inc, 2012) Dinc, Erdal; Baleanu, Dumitru; Ragno, Gaetano; Baleanu, Dumitru; De Luca, Michele; Ioele, Giuseppina; 6981
In this study a new signal processing approach based on the simultaneous use of fractional and continuous wavelet transforms was suggested for the quantitative estimation of melatonin and its photodegradation products in their samples. The UV spectra of melatonin and its degradation product were recorded between 200.0 and 455.0 nm and processed by the fractional wavelet algorithm. The obtained fractional wavelet coefficients were treated by biorthogonal and symplets wavelet families in order to quantify melatonic and its photodegradation product, respectively. The calibration graphs for the related main compound and its degradation product were obtained by measuring the fractional-continuous wavelet amplitudes at 432.0 nm for melatonin and 435.0 nm for its degradation product. The validity and applicability of the double transforms based on fractional and continuous wavelets were confirmed by analyzing various synthetic mixtures of melatonin and its degradation product. Successful results were reported in the application of the improved fractional-continuous wavelet transform to the sample analysis.
Multivariate analysis of paracetamol, propiphenazone, caffeine and thiamine in quaternary mixtures by PCR, PLS and ANN calibrations applied on wavelet transform data
(Pergamon-Elsevier Science LTD, 2008) Baleanu, Dumitru; Baleanu, Dumitru; Ioele, Giuseppina; De Luca, Michele; Ragno, Gaetano; 6981
The quantitative resolution of a quaternary pharmaceutical mixture consisting of paracetamol, propiphenazone, caffeine and thiamine was performed by the simultaneous use of fractional wavelet transform (FWT) with principal component regression (PCR), partial least squares (PLS) and artificial neural networks (ANN) methods. A calibration set consisting of 22 mixture solutions was prepared by means of an orthogonal experimental design and their absorption spectra were recorded in the spectral range of 210.0-312.3 nm and then transferred into the fractional wavelet domain and processed by FWT. The chemometric calibrations FWT-PCR, FWT-PLS and FWT-ANN were computed by using the relationship between the coefficients provided by FWT method and the concentration data from calibration set. An external validation was carried out by applying the developed methods to the analysis of synthetic mixtures of the related compounds, obtaining successful results. The models were finally used to assay the studied drugs in the commercial pharmaceutical formulations