Browsing by Author "Kasap, Esin Nagihan"

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Citation Count: Kasap, Esin Nagihan...et al., "Fast fluorometric enumeration of E. coli using passive chip", Journal of Microbiological Methods, Vol. 164, (2019).
Fast fluorometric enumeration of E. coli using passive chip
(Elsevier, 2019) Kasap, Esin Nagihan; Doğan, Üzeyir; Çoğun, Ferah; Yıldırım, Ender; Boyacı, İsmail Haklu; Çetin, Demet; Suludere, Zekiye; Tamer, Uğur; Ertaş, Nusret
In this report, a passive microfluidic chip design was developed for fast and sensitive fluorometric determination of Escherichia coli (E. coli) based on sandwich immunoassay. Initially, magnetic nanoparticles (MNPs) and chitosan modified mercaptopropionic acid capped cadmium telluride (CdTe) quantum dots (QDs) were functionalized with E.coli specific antibody to form a sandwich immunoassay with the E. coli. The magnetic separation and preconcentration of the E.coli from the sample solution was performed in the vial. Conjugation of QDs to the magnetically captured E. coli and washing were performed using a passive type of microchip. The microfluidic chip consists of four microchambers connected to each other by microchannels which act as capillary valves. Signal measurement was performed at the last chamber by using a hand-held spectrofluorometer equipped with a fiber optic reflection probe. The selectivity of the method was tested with Enterobacter aerogenes (E. aerogenes) and Salmonella enteritidis (S. enteritidis), it was observed that these bacteria have no interference effect on E.coli determination. The calibration curve was found to be linear in the range of 10(1)-10(5) cfu/mL with a correlation coefficient higher than 0.99. The limit of detection was calculated as 5 cfu/mL. The method was successfully applied to spiked tap and lake water samples. The results suggest that the developed method is applicable for on-site E. coli detection and offers several advantages such as large dynamic range, high sensitivity, high selectivity and short analysis time.
Citation Count: Ü. Doğan..et al., “Simultaneous Detection of Two Different Bacteria Using QDs and MNPs,” presented at the 10th Aegean Analytical Chemistry Days, (29 Eylül - 02 Ekim 2016), Çanakkale, Türkiye, 2016.
Simultaneous Detection of Two Different Bacteria Using QDs and MNPs
(2016) Doğan, Üzeyir; Kasap, Esin Nagihan; Çoğun, Ferah; Yıldırım, Ender; Çetin, Demet; Suludere, Zekiye; Boyacı, İsmail Hakkı; Ertaş, Nusret; Tamer, Uğur