Browsing by Author "Pamme, N."
Now showing 1 - 1 of 1
- Results Per Page
- Sort Options
Conference Object Citation - Scopus: 0Phaseguide assisted liquid lamination for magnetic bead-based assays(Chemical and Biological Microsystems Society, 2013) Phurimsak, C.; Yıldırım, Ender; Yildirim, E.; Trietsch, S.J.; Hankemeier, T.; Tarn, M.D.; Pamme, N.; Vulto, P.; Makine MühendisliğiWe demonstrate a simple, pump-free platform for performing rapid magnetic bead-based processes via their transfer through sequentially laminated liquid streams, made possible by the use of phaseguide technology. We have applied this strategy to two on-chip assays: (i) a streptavidin-biotin binding assay, and (ii) a sandwich immunoassay for the detection of C-reactive protein (CRP). Here, functionalized magnetic beads were pulled through alternating lanes of reagents and buffer solution, allowing multiple binding and washing processes to be reduced into a single step, significantly shortening procedural times compared to conventional multi-step bead-based assays.