Browsing by Author "Pamme, Nicole"

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Citation Count: Phurimsak, Chayakom...et all. "Phaseguide assisted liquid lamination for magnetic bead-based assays", 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 27-31 October 2013, Freiburg, Germany, pp. 1153-1155, 2013.
Phaseguide assisted liquid lamination for magnetic bead-based assays
(2013) Phurimsak, Chayakom; Yıldırım, Ender; Trietsch, Sebastiaan J.; Hankemeier, Thomas; Tarn, Mark D.; Pamme, Nicole; Vulto, Paul
We demonstrate a simple, pump-free platform for performing rapid magnetic bead-based processes via their transfer through sequentially laminated liquid streams, made possible by the use of phaseguide technology. We have applied this strategy to two on-chip assays: (i) a streptavidin-biotin binding assay, and (ii) a sandwich immunoassay for the detection of C-reactive protein (CRP). Here, functionalized magnetic beads were pulled through alternating lanes of reagents and buffer solution, allowing multiple binding and washing processes to be reduced into a single step, significantly shortening procedural times compared to conventional multi-step bead-based assays.
Citation Count: Phurimsak, C...et al. (2014). Phaseguide assisted liquid lamination for magnetic particle-based assays. Lab On A Chip, 14(13), 2334-2343. http://dx.doi.org/10.1039/c4lc00139g
Phaseguide assisted liquid lamination for magnetic particle-based assays
(Royal Soc Chemistry, 2014) Phurimsak, Chayakom; Yıldırım, Ender; Tarn, Mark D.; Trietsch, Sebastiaan J.; Hankemeier, Thomas; Pamme, Nicole; Vulto, Paul; 31835
We have developed a magnetic particle-based assay platform in which functionalised magnetic particles are transferred sequentially through laminated volumes of reagents and washing buffers. Lamination of aqueous liquids is achieved via the use of phaseguide technology; microstructures that control the advancing air-liquid interface of solutions as they enter a microfluidic chamber. This allows manual filling of the device, eliminating the need for external pumping systems, and preparation of the system requires only a few minutes. Here, we apply the platform to two on-chip strategies: (i) a one-step streptavidin-biotin binding assay, and (ii) a two-step C-reactive protein immunoassay. With these, we demonstrate how condensing multiple reaction and washing processes into a single step significantly reduces procedural times, with both assay procedures requiring less than 8 seconds.