Browsing by Author "Vulto, P."

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    Phaseguide Assisted Liquid Lamination for Magnetic Bead-Based Assays
    (Chemical and Biological Microsystems Society, 2013) Phurimsak, C.; Yıldırım, Ender; Yildirim, E.; Trietsch, S.J.; Hankemeier, T.; Tarn, M.D.; Pamme, N.; Vulto, P.; Makine Mühendisliği
    We demonstrate a simple, pump-free platform for performing rapid magnetic bead-based processes via their transfer through sequentially laminated liquid streams, made possible by the use of phaseguide technology. We have applied this strategy to two on-chip assays: (i) a streptavidin-biotin binding assay, and (ii) a sandwich immunoassay for the detection of C-reactive protein (CRP). Here, functionalized magnetic beads were pulled through alternating lanes of reagents and buffer solution, allowing multiple binding and washing processes to be reduced into a single step, significantly shortening procedural times compared to conventional multi-step bead-based assays.