Scopus İndeksli Yayınlar Koleksiyonu
Permanent URI for this collectionhttps://hdl.handle.net/20.500.12416/8651
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Article Citation - WoS: 32Citation - Scopus: 36Sers-Based Ultrafast and Sensitive Detection of Luteinizing Hormone in Human Serum Using a Passive Microchip(Elsevier Science Sa, 2018) Yildirim, Ender; Eryilmaz, Merve; Selbes, Yesim; Saglam, Necdet; Tamer, Ugur; Gjergjizi, Belma; Cogun, FerahHuman luteinizing hormone (LH) is an important analyte for doping control analysis since it increases the athletic performance. However, traditional methods to detect LH have few disadvantages, such as long analysis duration, waste disposal problem and sample matrix effect. Addressing these problems, surface enhanced Raman spectroscopy based LH analysis using a passive microfluidic chip was developed and optimized. Antibody modified magnetic gold nanoparticles captured the LH and then, 4-aminothiophenol (4-ATP) labeled nanoparticles formed the sandwich immunoassay structure. The complex and the other reactions occurred in different chambers of the chip. The SERS signals of 4-ATP were recorded from the chamber and the system was shown to detect 0.036 IU L-1 in serum samples. The performance of the immunoassay was compared to all other methods and the proposed assay was the fastest analysis of LH without any problems associated with the sensitivity. The shorter analysis time was recorded because the chip enables the control of all reactions in one place and there was no requirement of a specialized laboratory. (c) 2018 Elsevier B.V. All rights reserved.Article Citation - WoS: 30Citation - Scopus: 33Investigation on Replication of Microfluidic Channels by Hot Embossing(Taylor & Francis inc, 2017) Arikan, M. A. Sahir; Cogun, Ferah; Yildirim, Ender; Sahir Arikan, M.A.In this study, effects of embossing temperature, time, and force on production of a microfluidic device were investigated. Polymethyl methacrylate (PMMA) substrates were hot embossed by using a micromilled aluminum mold. The process parameters were altered to observe the variation of replication rate in width and depth as well as symmetry of the replicated microfluidic channels. Analysis of variance (ANOVA) on the experimental results indicated that embossing temperature was the most important process parameter, whereas embossing time and force have less impact. One distinguishing aspect of this study is that, the channels were observed to be skewed to either side of the channel depending on the location of the protrusions on the mold. The mechanism of the skewness was investigated by finite element analysis and discussed in detail. Results showed that the skewness depends on the flow characteristics of the material and could be reduced by increasing the embossing temperature. The best replication rates were obtained at parameter settings of 115 degrees C, 10kN, and 8min for the molds with minimum 56 mu m wide features of 120 mu m depth. We also showed that the fabricated channels could be successfully sealed by solvent-assisted thermo-compressive bonding at 85 degrees C under 5.5kN force.