Continuous Wavelet Transform Applied To the Quantitative Analysis of a Binary Mixture

Abstract

Signal processing methods, continuous wavelet transform (CWT) methods, were proposed for the simultaneous spectral determination of ampicillin sodium (AS) and sulbactam sodium (SS) in their synthetic binary mixtures and pharmaceutical preparations. Firstly, the ratio spectra were obtained by dividing the absorption spectra of a pure compound and its binary mixture by a standard spectrum of one of the compounds in binary mixture. Secondly, the ratio spectra of AS, SS and their samples were processed by the Mexican hat-CWT and Symtles3-CWT (MEX-CWT and SYM3-CWT, respectively). hi this application, the MEX and SYM3 among wavelet families were found to be optimal to obtain the highest spectral recoveries. Calibration graphs of AS and SS in the linear concentration range of 10-70 mu g/mL and 10-55 mu g/mL were obtained by measuring the amplitudes of the transformed ratio signals, respectively. Percent mean recoveries obtained by application of the CWT methods to the synthetic mixtures were found between 99.1 % and 103.6 %. Both SYM3-CWT and MEX-CWT methods were applied to the real samples containing two active compounds and successful results were obtained.