Spectrophotometric Simultaneous Determination of Atorvastatin-Amlodipin and Telmisartan-Hidrochlorothiazide Mixtures by Bivariate and Multivariate Calibrations

Abstract

The bivariate and multivariate spectral calibrations were proposed for the simultaneous quantitative analysis of two different mixtures containing atorvastatin-amlodipine and telmisartan- hydrochlorothiazide combinations in tablets. These methods do not require any separation step of the related compounds in the same mixtures. In the first case, the bivariate spectral calibration (BC) was obtained by using four linear regression functions with two single-component regressions for each compound at the two-wavelength sets 218, 260 nm for ATO-AML mixture and 224, 236 nm for TEL-HCT mixture. In the second case, the multivariate spectral calibration (MC) was performed by the simultaneous use of 26 linear regression equations with 26 single-component regression for each compound at a 26-wavelength set from 218 nm to 268 nm with the intervals of Delta lambda = 2 nm for ATO-AML mixture and at a 26-wavelength set from 220 nm to 320 nm with the intervals of Delta lambda = 4 nm for TEL-HCT mixture. All the methods were validated by analysing the synthetic mixture of two active compounds and by using standard addition technique. The obtained results were successfully compared with each other and both methods were successfully applied to the quantitative evaluation of two different mixtures consisting of ATO-AML and TEL-HCT.