A Signal Processing Tool Based on the Continuous Wavelet for the Simultaneous Determination of Estradiol Valerate and Cyproterone Acetate in Their Mixtures

Abstract

A signal processing tool based on the continuous wavelet transform (CWT) was proposed for the simultaneous determination of estradiol valerate (EV) and cyproterone acetate (CA) in their mixtures. Different continuous wavelet families were tested in order to find the optimum family of the wavelets. The Symlets 8 continuous wavelet transform (SYM8-CWT) was observed to be the optimal one. The SYM8-CWT approach was applied to the absorption spectra of the related compounds in the spectral range of 210.00-312.35 nm for the calibration solutions of the compounds in the concentration range of 10-50 mu g/mL for EV and 5-30 mu g/mL for CA. The calibration equations for EA and CA were computed by measuring the continuous wavelet signals at 260.75 nm and 238.40 nm, respectively. The proposed method was validated by the independent analysis of the synthetic mixtures and by the standard addition technique. It was observed that the selectivity and the sensitivity in the application of the db5-CWT method to the determination of EV and CA compounds were satisfactory